Anti-CDA antibody
| Name | Anti-CDA antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab82347 |
| Prices | $384.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide selected from the Center region of human CDA conjugated to KLH |
| Description | Rabbit Polyclonal |
| Gene | CDA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab82347 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82347,10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-CDA antibody (ab82347) at 1/50 dilution + HeLa cell line lysate at 35 µg
Anti-CDA antibody (ab82347) at 1/50 dilution + mouse kidney tissue lysates at 35 µg