All lanes : Anti-CDA antibody - C-terminal (ab82346) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Mouse kidney tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution
ab82346 staining CDA in murine pancreatic adenocarcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 5% serum for 1 hour at 25°C and then incubated with ab82346 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an undiluted HRP conjugated horse anti-rabbit polyclonal.See Abreview
ab82346 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab82346 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.