All lanes : Anti-CDAN1 antibody (ab31236) at 0.5 µg/mlLane 1 : Hela B Cell Lysate at 30 µgLane 2 : Hela B Cell Lysate at 60 µg
ICC/IF image of ab31236 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31236, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Anti-CDAN1 antibody (ab31236) at 1 µg/ml + Ovary (Human) Tissue Lysate - adult normal tissue (ab30222) at 20 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.