Anti-Cdc20 antibody
| Name | Anti-Cdc20 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab26483 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Chicken, Pig |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Cdc20 |
| Description | Rabbit Polyclonal |
| Gene | CDC20 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Cdc20 antibody (ab26483) at 1 µg/mlLane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab7908)Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913)Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 4 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate (ab7914)Lysates/proteins at 20 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
ICC/IF image of ab26483 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab26483, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Product References
The anaphase-promoting complex or cyclosome supports cell survival in response to - The anaphase-promoting complex or cyclosome supports cell survival in response to
Chen M, Gutierrez GJ, Ronai ZA. PLoS One. 2012;7(4):e35520.
Cyclin-dependent kinase-1 (Cdk1)/cyclin B1 dictates cell fate after mitotic - Cyclin-dependent kinase-1 (Cdk1)/cyclin B1 dictates cell fate after mitotic
Sakurikar N, Eichhorn JM, Chambers TC. J Biol Chem. 2012 Nov 9;287(46):39193-204.
Androgen receptor regulates a distinct transcription program in - Androgen receptor regulates a distinct transcription program in
Wang Q, Li W, Zhang Y, Yuan X, Xu K, Yu J, Chen Z, Beroukhim R, Wang H, Lupien M, Wu T, Regan MM, Meyer CA, Carroll JS, Manrai AK, Janne OA, Balk SP, Mehra R, Han B, Chinnaiyan AM, Rubin MA, True L, Fiorentino M, Fiore C, Loda M, Kantoff PW, Liu XS, Brown M. Cell. 2009 Jul 23;138(2):245-56.
Evidence that mitotic exit is a better cancer therapeutic target than spindle - Evidence that mitotic exit is a better cancer therapeutic target than spindle
Huang HC, Shi J, Orth JD, Mitchison TJ. Cancer Cell. 2009 Oct 6;16(4):347-58.
The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for - The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for
Nilsson J, Yekezare M, Minshull J, Pines J. Nat Cell Biol. 2008 Dec;10(12):1411-20.
Cyclin E overexpression impairs progression through mitosis by inhibiting - Cyclin E overexpression impairs progression through mitosis by inhibiting
Keck JM, Summers MK, Tedesco D, Ekholm-Reed S, Chuang LC, Jackson PK, Reed SI. J Cell Biol. 2007 Jul 30;178(3):371-85.