All lanes : Anti-Cdc20 antibody (ab183479) at 1/2000 dilutionLane 1 : U2OS cells transfected with control si RNALane 2 : U2OS cells transfected with Cdc20 si RNALane 3 : Rat INS1 cell lysateLane 4 : Mouse NIH 3T3 cell lysateLysates/proteins at 40 µg per lane.
Western blot analysis of Immunoprecipitation using U2OS cellsLane 1: Input.Lane 2: control serum.Lane 3: ab183479.
Immunofluorescent analysis of U2OS cells showing labeling of Cdc20 with ab183479 at 1/1000 dilution. Anti-Cyclin B labeling is seen in red and DAPI counterstain in blue. Lower image is merged.