Anti-Cdc25B antibody (ab70927) at 1/50 dilution + Ramos cell line lysates at 12.5 µg
Formalin fixed, paraffin embedded human breast carcinoma tissue reacted with a70927 at a 1/10 dilution, by Immunohistochemistry. ab70927 was peroxidase conjugated to the secondary antibody, followed by DAB staining.
Anti-Cdc25B antibody (ab70927) at 1/100 dilution + Active human Cdc25B full length protein (ab105906) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds