All lanes : Anti-Cdc37 antibody (ab61773) at 1/500 dilutionLane 1 : extracts from NIH/3T3 cells with no peptideLane 2 : extracts from NIH/3T3 cells with peptide
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab61773 at a dilution of 1/50. Left: untreated sample. Right: sample treated with immunising peptide.
All lanes : Anti-Cdc37 antibody (ab61773) at 1/1000 dilutionLane 1 : Human Cdc37 full length protein (ab78881) at 0.1 µgLane 2 : Human Cdc37 full length protein (ab78881) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds