Anti-CDC45L antibody [EPR5759]

• Supplier: Abcam
• Catalog: ab126762
• Prices: $313.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5759
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to residues in Human CDC45L
• Description: Rabbit Monoclonal
• Gene: CDC45
• Conjugate: Unconjugated

Product images

ICC/IF image of ab126762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126762 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

All lanes : Anti-CDC45L antibody [EPR5759] (ab126762) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Jurkat cell lysateLane 3 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit at 1/2000 dilution

ab126762, at 1/100 dilution, staining CDC45L in paraffin-embedded Human colon tissue by Immunohistochemistry.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD