All lanes : Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : U87-MG cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution
Immunofluorescent analysis of U87-MG cells labeling CDK5RAP3 with ab168353 at 1/50 dilution.
Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution + immunoprecipitation pellet from 293T cell lysate SecondaryHRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG