Anti-CDT1 antibody
| Name | Anti-CDT1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab83174 |
| Prices | $387.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human CDT1 |
| Description | Rabbit Polyclonal |
| Gene | CDT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-CDT1 antibody (ab83174) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab83174 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83174, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
Product References
Exploring the spectroscopic differences of Caki-2 cells progressing through the - Exploring the spectroscopic differences of Caki-2 cells progressing through the
Jimenez-Hernandez M, Hughes C, Bassan P, Ball F, Brown MD, Clarke NW, Gardner P. Analyst. 2013 Jul 21;138(14):3957-66.
S-phase sensing of DNA-protein crosslinks triggers TopBP1-independent ATR - S-phase sensing of DNA-protein crosslinks triggers TopBP1-independent ATR
Wong VC, Cash HL, Morse JL, Lu S, Zhitkovich A. Cell Cycle. 2012 Jul 1;11(13):2526-37.