All lanes : Anti-CEBP gamma antibody (ab54899) at 1/1000 dilutionLane 1 : Mouse adipocyte lysate - untreatedLane 2 : Mouse adipocyte lysate - 5nm rapamycin treatment for 48 hoursLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated anti-mouse IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under non-reducing conditions.Observed band size : 47 kDa (why is the actual band size different from the predicted?)Exposure time : 30 secondsThis image is courtesy of an Abreview submitted by Kun DongSee Abreview