All lanes : Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185) at 1/5000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185) at 1/1000 dilutionLane 1 : U87-MG cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling CECR5 with ab184185. Right lane shows PBS negative control.
Immunofluorescent analysis of HeLa cells labeling CECR5 with ab184185 at a 1/100 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).