All lanes : Anti-CEECAM1 antibody [EPR13744] (ab181979) at 1/10000 dilutionLane 1 : Human fetal brain lysateLane 2 : Jurkat cell lysateLane 3 : 293 cell lysateLane 4 : U87-MG cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling CEECAM1 with ab181979 at 1/300 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (greeen), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.