Anti-CEMP1 antibody
| Name | Anti-CEMP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab134231 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to a region within C terminal amino acids 136-185 (RHFLNWALQQ REENSGRARR VPPVPRTAPV SKGEGSHPPQ NSNGEKVKTI) of Human CEMP1 (NP_001041677) Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | CEMP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab134231 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab134231 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-CEMP1 antibody (ab134231) at 1 µg/ml + HeLa cell lysate at 10 µg