Anti-CENPA antibody [EP800Y] - Centromere Marker

• Supplier: Abcam
• Catalog: ab45694
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP800Y
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues in the C-terminal of human CENPA
• Description: Rabbit Monoclonal
• Gene: CENPA
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab45694 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45694, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694) at 1/20000 dilution + HeLa cell lysate (10ug/lane)

Immunofluorescent staining of HeLa cells using ab45694 (1:250).

IHC image of ab45694 staining CENPA in Human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45694, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ab45694 showing positive staining in Colonic adenocarcinoma tissue.

ab45694 showing positive staining in Lymphoma tissue.

ab45694 showing positive staining in Prostatic carcinoma tissue.

ab45694 showing positive staining in Lung carcinoma tissue.