Anti-CENPA antibody - ChIP Grade
| Name | Anti-CENPA antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab33565 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ChIP |
| Species Reactivities | Mouse, Hamster |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Mouse CENPA |
| Description | Rabbit Polyclonal |
| Gene | Cenpa |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : MarkerLanes 2 - 3 : Anti-CENPA antibody - ChIP Grade (ab33565) at 1 µg/mlLane 1 : As aboveLane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179) at 20 µgLane 3 : MEF1 at 20 µgSecondaryLanes 2 - 3 : IRDye 680 conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
ICC/IF image of ab33565 stained MEF1 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab33565, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Product References
The histone variant composition of centromeres is controlled by the pericentric - The histone variant composition of centromeres is controlled by the pericentric
Boyarchuk E, Filipescu D, Vassias I, Cantaloube S, Almouzni G. J Cell Sci. 2014 Aug 1;127(Pt 15):3347-59.
Non-coding murine centromeric transcripts associate with and potentiate Aurora B - Non-coding murine centromeric transcripts associate with and potentiate Aurora B
Ferri F, Bouzinba-Segard H, Velasco G, Hube F, Francastel C. Nucleic Acids Res. 2009 Aug;37(15):5071-80.