Anti-CENPF antibody
| Name | Anti-CENPF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5 |
| Prices | $403.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FC ICC/IF ICC/IF ICC/IF IHC-P WB IP |
| Species Reactivities | Mouse, Human |
| Antigen | Fusion protein with C-terminus of CENP-F (Human) |
| Description | Rabbit Polyclonal |
| Gene | CENPF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab5 staining CENPF in Mouse embryonic fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% BSA for 30 minutes at 23°C. Samples were incubated with primary antibody (1/300 in PBS + 0.1% Triton-X 100 + 1% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview
HeLa cells were labelled with anti-ACA and anti-CENPF (ab5). ab5 was used at a working dilution of 1/400. This image demonstrates the dramatic increase in fluorescence that occurs late in G2 cells (indicated by arrows). In the final panel DAPI is pseudo-coloured blue, while ACA and CENPF are coloured green and red respectively. 40x magnification.
Interpahse and Prophase HeLa cells were labelled with anti-ACA and anti-CENPF (ab5). ab5 was used at a working dilution of 1/400. This image emphasizes the redistribution of CENPF from the nuclear matrix during late G2 following entry into the initial stages of mitosis (see the accompanying image). Distinct punctate CENPF patterns proximally located in relation to the centromeres can be seen. In the final panel DAPI is pseudo-coloured blue, while ACA and CENPF are green and red respectively. 100x magnification.
ICC/IF image of ab5 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab5, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
All lanes : Anti-CENPF antibody (ab5) at 1/1500 dilutionLane 1 : MitoticLane 2 : Asyn.Lane 3 : 50gamma Mitotic HeLaLane 4 : 50gamma Asyn. HeLa lys
ab5 staining (human) T98G glioma cells by flow cytometry. Cells were trypsinized, spun down & resuspended in PBS before fixing and permeabilised in ethanol. The primary antibody was diluted 1/400 and incubated with the cells for 2 hours at 20°C. An Alexa Fluor® 488 conjugaetd goat anti-rabit IgG was used as the secondary antibody. Gating strategy: intact cells with >80% and <250% of G1 DNA contentSee Abreview
Ab5 staining Human normal colon tissue. Staining is localised to nuclear compartment.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Differential role of nonhomologous end joining factors in the generation, DNA - Differential role of nonhomologous end joining factors in the generation, DNA
Felgentreff K, Du L, Weinacht KG, Dobbs K, Bartish M, Giliani S, Schlaeger T, DeVine A, Schambach A, Woodbine LJ, Davies G, Baxi SN, van der Burg M, Bleesing J, Gennery A, Manis J, Pan-Hammarstrom Q, Notarangelo LD. Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8889-94. doi:
Differential sensitivity of Glioma stem cells to Aurora kinase A inhibitors: - Differential sensitivity of Glioma stem cells to Aurora kinase A inhibitors:
Mannino M, Gomez-Roman N, Hochegger H, Chalmers AJ. Stem Cell Res. 2014 Jul;13(1):135-43.
The efficiency of homologous recombination and non-homologous end joining systems - The efficiency of homologous recombination and non-homologous end joining systems
Bee L, Fabris S, Cherubini R, Mognato M, Celotti L. PLoS One. 2013 Jul 11;8(7):e69061.
Highly sensitive automated method for DNA damage assessment: gamma-H2AX foci - Highly sensitive automated method for DNA damage assessment: gamma-H2AX foci
Hernandez L, Terradas M, Martin M, Tusell L, Genesca A. Int J Mol Sci. 2013 Jul 30;14(8):15810-26.
The complexity of DNA double strand breaks is a critical factor enhancing - The complexity of DNA double strand breaks is a critical factor enhancing
Yajima H, Fujisawa H, Nakajima NI, Hirakawa H, Jeggo PA, Okayasu R, Fujimori A. DNA Repair (Amst). 2013 Nov;12(11):936-46.
Indicators of replicative damage in equine tendon fibroblast monolayers. - Indicators of replicative damage in equine tendon fibroblast monolayers.
Rich T, Henderson LB, Becker DL, Cornell H, Patterson-Kane JC. BMC Vet Res. 2013 Sep 11;9:180.
Cohesin phosphorylation and mobility of SMC1 at ionizing radiation-induced DNA - Cohesin phosphorylation and mobility of SMC1 at ionizing radiation-induced DNA
Bauerschmidt C, Woodcock M, Stevens DL, Hill MA, Rothkamm K, Helleday T. Exp Cell Res. 2011 Feb 1;317(3):330-7.
Ki-67 expression is superior to mitotic count and novel proliferation markers - Ki-67 expression is superior to mitotic count and novel proliferation markers
Ladstein RG, Bachmann IM, Straume O, Akslen LA. BMC Cancer. 2010 Apr 14;10:140.
Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere - Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere
Gurden MD, Holland AJ, van Zon W, Tighe A, Vergnolle MA, Andres DA, Spielmann HP, Malumbres M, Wolthuis RM, Cleveland DW, Taylor SS. J Cell Sci. 2010 Feb 1;123(Pt 3):321-30.
Cohesin promotes the repair of ionizing radiation-induced DNA double-strand - Cohesin promotes the repair of ionizing radiation-induced DNA double-strand
Bauerschmidt C, Arrichiello C, Burdak-Rothkamm S, Woodcock M, Hill MA, Stevens DL, Rothkamm K. Nucleic Acids Res. 2010 Jan;38(2):477-87.