All lanes : Anti-Centaurin beta 2 [EPR15721] antibody (ab191424) at 1/5000 dilutionLane 1 : HeLa lysateLane 2 : Jurkat lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Flow Cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling Centaurin beta 2 with ab191424 at 1/270 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
All lanes : Anti-Centaurin beta 2 [EPR15721] antibody (ab191424) at 1/5000 dilutionLane 1 : 293 lysateLane 2 : MOLT4 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Centaurin beta 2 [EPR15721] antibody (ab191424) at 1/5000 dilutionLane 1 : Human fetal lung lysateLane 2 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-Centaurin beta 2 [EPR15721] antibody (ab191424) at 1/1000 dilutionLane 1 : RAW 264.7 lysateLane 2 : PC12 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution