Adult rat was perfused with 4% PFA in 0.1M PBS. Fluorescent staining was performed in duodenum sections with anti-Ceruloplasmin (ab110449) primary and Alexa 488 conjugated secondary antibody. This antibody stained gut enterocytes. Nucleus stained with Hoechst at high power magnification. See Abreview
All lanes : Anti-Ceruloplasmin antibody (ab110449) at 1 µg/mlLane 1 : Human Plasma Total Protein Lysate Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Anti-Ceruloplasmin antibody (ab110449) at 1 µg/ml + Human Ceruloplasmin full length protein (ab77930) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds