Anti-Choline Acetyltransferase antibody
| Name | Anti-Choline Acetyltransferase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab18736 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Sheep |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Rabbit, Guinea Pig, Human, Pig, Chicken |
| Antigen | Synthetic peptide: GLFSSYRLPGHTQDTLVAQKSS conjugated to KLH, corresponding to amino acids 167-188 of Pig Choline Acetyltransferase |
| Description | Sheep Polyclonal |
| Gene | Chat |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab18736 staining Choline Acetyltransferase in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100 and blocked with 3% donkey serum in 0.3% Triton X-100/TBS for 1 hour at 21°C. The sample was incubated with primary antibody (1/1000 in 1% donkey serum + 0.3% Triton X-100/TBS) at 4°C for 24 hours. An Alexa Fluor® 594-conjugated donkey anti-sheep polyclonal (1/200) was used as the secondary antibody.See Abreview
Immunohistochemical detection of ChAT in rat basal forebrain using Sheep anti ChAT (ab18736).Rat was perfused with 1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min. and then fixed with 4% formaldehyde containing 15% picric acid in 0.1M TBS, pH, 7.4 for 30 min. The brain was then cryoprotected in 30% overnight and cut into 40 nm free-floating sections. Sections were washed 2x with TBS for 20 min. and passed through asending ethanol and finally transferred to 0.1 M TBS for rehydration for 1h.
Product References
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