Anti-Choline Acetyltransferase antibody

• Supplier: Abcam
• Catalog: ab68779
• Prices: $385.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P IHC-F ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Guinea Pig, Dog, Human, Pig
• Antigen: A synthetic peptide as a part of human, rat and mouse Choline Acetyltransferase conjugated to an immunogenic carrier protein
• Description: Rabbit Polyclonal
• Gene: Chat
• Conjugate: Unconjugated

Product images

ICC/IF image of ab68779 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68779, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI (ab104139) was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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