Anti-Chromogranin A antibody
| Name | Anti-Chromogranin A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab45179 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Dog, Human, Pig, Monkey |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Chromogranin A aa 1-100 (N terminal) |
| Description | Rabbit Polyclonal |
| Gene | CHGA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Chromogranin A antibody (ab45179) at 1/10000 dilution (unpurified) + PC-12 cell lysate at 10 µgSecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilutionObserved band size : 86 kDa (why is the actual band size different from the predicted?)
ICC/IF image of unpurified ab45179 stained PANC-1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45179, 1/50 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Unpurified ab45179 staining Chromogranin A in pig small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/750 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.See Abreview
Chromogranin A staining on monkey pancreas sections. The sections were subjected to heat mediated epitope retrieval using citric acid (pH 6) and blocked using 1% BSA for 10 mins at 21°C. Unpurifeid ab45179 was used at a dilution of 1/750 using TBS/BSA/Azide for 2 hours at 21°C. (Red arrowheads indicate extra Islet positive cells).See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prancreatic tissue labelling Chromogranin A with unpurified ab45179 at a 1/100 dilution.
Product References
Chlamydia trachomatis antigens in enteroendocrine cells and macrophages of the - Chlamydia trachomatis antigens in enteroendocrine cells and macrophages of the
Dlugosz A, Tornblom H, Mohammadian G, Morgan G, Veress B, Edvinsson B, Sandstrom G, Lindberg G. BMC Gastroenterol. 2010 Feb 16;10:19.
Chlamydia trachomatis antigens in enteroendocrine cells and macrophages of the - Chlamydia trachomatis antigens in enteroendocrine cells and macrophages of the
Dlugosz A, Tornblom H, Mohammadian G, Morgan G, Veress B, Edvinsson B, Sandstrom G, Lindberg G. BMC Gastroenterol. 2010 Feb 16;10:19.