Anti-Chromogranin B antibody

• Supplier: Abcam
• Catalog: ab12242
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-F ICC/IF IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: C-terminal 15 amino acids of chromogranin B conjugated to ovalbumin
• Description: Rabbit Polyclonal
• Gene: CHGB
• Conjugate: Unconjugated

Product images

ab12242(1:1000) staining Chromogranin B in human pancreas (IgG inset) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Chromogranin B in secretory compartments of the pancreas. Insert panel depicts negative control (no primary antibody).Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Product References

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