All lanes : Anti-CHST3 antibody [EPR15789] (ab193359) at 1/1000 dilutionLane 1 : 293 cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-CHST3 antibody [EPR15789] (ab193359) at 1/1000 dilution + Raw264.7 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling CHST3 with ab193359 at 1/350 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.