Anti-CITED4 antibody (ab77018) at 5 µg/ml + immunogen at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilutionObserved band size : 32 kDa (why is the actual band size different from the predicted?)
All lanes : Anti-CITED4 antibody (ab77018) at 5 µg/mlLane 1 : CITED4 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionObserved band size : 23 kDa (why is the actual band size different from the predicted?)
All lanes : Anti-CITED4 antibody (ab77018) at 1/1000 dilutionLane 1 : Whole tissue lysate prepared from mouse liverLane 2 : Whole tissue lysate prepared from mouse brainLysates/proteins at 10 µg per lane.SecondaryHRP conjugated horse anti-mouse IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under non-reducing conditions.Observed band size : 21 kDa (why is the actual band size different from the predicted?)Additional bands at : 38 kDa (possible non-specific binding),50 kDa (possible non-specific binding).Exposure time : 1 hourThis image is a courtesy of an anonymous AbreviewSee Abreview