Anti-CK068 antibody [46.2_2D8]

• Supplier: Abcam
• Catalog: ab127973
• Prices: $370.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 46.2_2D8
• Applications: WB IP ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: HepG2 cells
• Description: Mouse Monoclonal
• Gene: C11orf68
• Conjugate: Unconjugated

Product images

ab127973 at 1/100 dilution staining CK068 in 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells by Immunocytochemistry.

Lanes 1 & 5 : MWt markerLanes 2 - 4 : Anti-CK068 antibody [46.2_2D8] (ab127973) at 1/1000 dilutionLanes 6 - 8 : V5 antibody controlLane 1 : As aboveLane 2 : Transfected HeLa cell lysateLane 3 : Non-transfected HeLa cell lysateLane 4 : SH-SY5Y cell lysateLane 5 : As aboveLane 6 : Transfected HeLa cell lysateLane 7 : Non-transfected HeLa cell lysateLane 8 : SH-SY5Y cell lysate

2 µg of ab127973 was added to aliquots of cleared cell lysates from transfected Human cell lines and incubated for 2 hr at 4°C. Protein-G Dynabeads were then added, and lysates were incubated for additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody was excluded. Lane 1: Molecular weight marker Lane 2: Cell Lysate control Lane 3: Cell lysate with MAb Lane 4: Cell lysate without MAb Lane 5: V5 tag control Lane 6: Molecular weight marker