Anti-CLASP1 antibody [EPR3409]

Name	Anti-CLASP1 antibody [EPR3409]
Supplier	Abcam
Catalog	ab108620
Prices	$391.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR3409
Applications	ICC/IF ICC/IF WB IHC-P FC
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CLASP1 aa 1-100
Description	Rabbit Monoclonal
Gene	CLASP1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-CLASP1 antibody [EPR3409] (ab108620) at 1/20000 dilution (purified)Lane 1 : U87-MG cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-CLASP1 antibody [EPR3409] (ab108620) at 1/50000 dilution (purified) + Rat brain tissue lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-CLASP1 antibody [EPR3409] (ab108620) at 1/10000 dilution (unpurified)Lane 1 : T47-D cell lysateLane 2 : C6 cell lysateLysates/proteins at 10 µg per lane.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling CLASP1 with purified ab108620 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling CLASP1 with purified ab108620 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CLASP1 with unpurified ab108620 at 1/250.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CLASP1 (green) with ab108620 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/100) and secondary antibody, ab150120 Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CLASP1 with unpurified ab108620 at 1/100.

Flow cytometry analysis of HeLa cells labelling CLASP1 with purified ab108620 at 1/50 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Overlay histogram showing HeLa cells stained with unpurified ab108620 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108620, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Product References

CLIP-170 recruits PLK1 to kinetochores during early mitosis for chromosome - CLIP-170 recruits PLK1 to kinetochores during early mitosis for chromosome

Amin MA, Itoh G, Iemura K, Ikeda M, Tanaka K. J Cell Sci. 2014 Jul 1;127(Pt 13):2818-24.

Plus-end tracking proteins, CLASPs, and a viral Akt mimic regulate - Plus-end tracking proteins, CLASPs, and a viral Akt mimic regulate

Naghavi MH, Gundersen GG, Walsh D. Proc Natl Acad Sci U S A. 2013 Nov 5;110(45):18268-73. doi:

Analysis of substrates of protein kinase C isoforms in human breast cells by the - Analysis of substrates of protein kinase C isoforms in human breast cells by the

Chen X, Zhao X, Abeyweera TP, Rotenberg SA. Biochemistry. 2012 Sep 11;51(36):7087-97. Epub 2012 Aug 29.