Anti-Claudin 2 antibody
| Name | Anti-Claudin 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53032 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Mouse, Human, Rat |
| Antigen | A synthetic peptide derived from human Claudin 2 |
| Description | Rabbit Polyclonal |
| Gene | CLDN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Claudin 2 antibody (ab53032) at 1/500 dilutionLane 1 : NIH 3T3 cell extractLane 2 : NIH 3T3 cell extract with immunising peptide
ICC/IF image of ab53032 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53032, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.