Anti-Claudin 23 antibody (ab23355) at 1 µg/ml + HepG2 cell lysate
All lanes : Anti-Claudin 23 antibody (ab23355) at 1 µg/mlLane 1 : Y79 (Human retinoblastoma cell line) Whole Cell LysateLane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
ab23355 staining paraffin embedded human placenta tissue at 5 ug/ml.
ab23355 staining Claudin 23 in kidney cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with methanol, permeabilized with Triton X-100 and blocked with 5% BSA for 30 minutes. Samples were incubated with primary antibody (1/600 in PBS + 0.5% BSA + 0.1% Triton X-100) for 1 hour at 20°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/800) was used as the secondary antibody. See Abreview