Anti-Claudin 4 antibody
| Name | Anti-Claudin 4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53156 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human, Mouse, Rat, Pig |
| Antigen | Synthetic peptide (Human) |
| Description | Rabbit Polyclonal |
| Gene | CLDN4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Claudin 4 antibody (ab53156) at 1/500 dilutionLane 1 : extracts from HeLa cells, untreated.Lane 2 : extracts from HeLa cells, treated with the immunizing peptide.
Ab53156 staining Human colon. Staining is localised to the cell membrane.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ICC/IF image of ab53156 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53156, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Claudin-4 expression in gastric cancer cells enhances the invasion and is - Claudin-4 expression in gastric cancer cells enhances the invasion and is
Hwang TL, Changchien TT, Wang CC, Wu CM. Oncol Lett. 2014 Sep;8(3):1367-1371. Epub 2014 Jun 27.
Protein expression signatures for inhibition of epidermal growth factor - Protein expression signatures for inhibition of epidermal growth factor
Myers MV, Manning HC, Coffey RJ, Liebler DC. Mol Cell Proteomics. 2012 Feb;11(2):M111.015222.
Claudin-3 and Clara cell 10 kDa protein as early signals of cigarette - Claudin-3 and Clara cell 10 kDa protein as early signals of cigarette
Cuzic S, Bosnar M, Kramaric MD, Ferencic Z, Markovic D, Glojnaric I, Erakovic Haber V. Toxicol Pathol. 2012 Dec;40(8):1169-87.