Anti-CLCN3 antibody (ab28736) at 1.25 µg/ml + HepG2 cell lysateSecondaryHRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000
Immunohistochemisty analysis of human brain tissue labelling CLCN3 with ab28736.
ICC/IF image of ab28736 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28736, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-CLCN3 antibody (ab28736) at 2.5 µg/mlLane 1 : HepG2 cell lysateLane 2 : HepG2 cell lysate with blocking peptide at 2 µg/mlLysates/proteins at 25 µg per lane.