All lanes : Anti-CLN8 antibody [EPR15048] (ab187655) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-CLN8 antibody [EPR15048] (ab187655) at 1/1000 dilutionLane 1 : rat PC12 cell lysateLane 2 : mouse NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human thyroid carcinoma tissue labeling CLN8 with ab187655 at a 1/100 dilution. Counter stained with hematoxylin.
Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling CLN8 with ab187655 at a 1/250 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).