All lanes : Anti-CNNM4 antibody (ab191207) at 0.4 µg/mlLane 1 : HeLa whole cell lysate prepared using RIPALane 2 : 293T whole cell lysate prepared using RIPALane 3 : Jurkat whole cell lysate prepared using RIPALane 4 : mouse TMCM-1 whole cell lysate prepared using NETNLane 5 : mouse NIH 3T3 whole cell lysate prepared using NETNLysates/proteins at 50 µg per lane.developed using the ECL technique
Immunoprecipitation analysis of HeLa whole cell lysate prepared using RIPA lysis buffer (1.0 mg per IP reaction; 20% of IP loaded) using ab191207 used at 6 µg per reaction lane 1. Lane 2 shows control IgG. For blotting ab191207 was used at 1 µg/ml. Chemiluminescence with an exposure time of 30 seconds.