Western blot detection of Chk2 phosphorylation using nocodazole-treated HeLa cells. Blots were probed with Chk2 (B-4): sc-17748 (A) and p-Chk2 (Thr 68)-R: sc-16297-R (B, C). In B and C, the antibody was preincubated with cognate phosphorylated or unphosphorylated peptide, respectively.
p-Chk2 (Thr 68)-R: sc-16297-R. Western blot analysis of Chk2 phosphorylation in HL-60 (A) and PMA-treated HL-60 (B) whole cell lysates.
p-Chk2 (Thr 68)-R: sc-16297-R. Western blot analysis of Cdc2 p34 phosphorylation in untreated (A), UV irradiated (B) and UV irradiated and lambda protein phosphatase treated (C) HeLa whole cell lysates.
Western blot analysis of Chk2 phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-Chk2 (Thr 68)-R: sc-16297-R (A,B,C) and Chk2 (C-18): sc-8813 (D,E,F).