All lanes : Anti-CNPY2 antibody [EPR14056-39] (ab181215) at 1/10000 dilutionLane 1 : MCF7 cell line lysateLane 2 : HeLa cell line lysateLane 3 : HepG2 cell line lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CNPY2 with ab181215 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling CNPY2 with ab181215 at 1/30 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181215 at 1/50 dilution Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling CNPY2 with ab181215 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.