All lanes : Anti-CNTF Receptor alpha antibody (ab58560) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) at 20 µgLane 2 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711) at 20 µgLane 3 : Skeletal Muscle (Rat) Tissue Lysate - normal tissue (ab29376) at 20 µgLane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgLane 5 : Brain (Mouse) Tissue Lysate (ab27253) at 10 µgLane 6 : Brain (Rat) Tissue Lysate (ab7942) at 10 µgSecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution