All lanes : Anti-COCH antibody (ab171410) at 1/100 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : COCH-transfected 293 cell lysateLysates/proteins at 2 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle, labeling COCH with ab171410 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HeLa cells labeling COCH with ab171410 at 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.