All lanes : Anti-COG7 antibody [EPR9942] (ab168362) at 1/1000 dilutionLane 1 : MOlT4 cell lysateLane 2 : HeLa cell lysateLane 3 : MCF7 cell lysateLane 4 : C6 cell lysateLane 5 : RAW 264.7 cell lysateLane 6 : PC12 cell lysateLane 7 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling COG7 with ab168362 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COG7 with ab168362 at 1/100 dilution.
Immunofluorescent analysis of HeLa cells labeling COG7 with ab168362 at 1/100 dilution. DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized HeLa cells labeling COG7 (red) with ab168362 at 1/10 dilution, compared to a nonspecific control antibody (green).