All lanes : Anti-COL23A1 antibody [EPR6066(2)(B)] (ab168356) at 1/1000 dilutionLane 1 : Fetal brain lysateLane 2 : Fetal lung lysateLane 3 : NCI-H460 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution
Anti-COL23A1 antibody [EPR6066(2)(B)] (ab168356) at 1/1000 dilution + mouse brain lysate at 10 µgSecondaryHRP labeled goat anti-rabbit at 1/2000 dilution
Anti-COL23A1 antibody [EPR6066(2)(B)] (ab168356) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate SecondaryHRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG