Anti-Collagen I antibody
| Name | Anti-Collagen I antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab34710 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ELISA WB ELISA IHC-P ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Goat, Horse, Bovine, Human, Pig |
| Antigen | Full length native protein (purified) corresponding to Human Collagen I aa 1-1464 |
| Description | Rabbit Polyclonal |
| Gene | COL1A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab34710 staining Collagen I in Pig small intestines tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.See Abreview
ab34710 at 1/500 staining human adrenal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was blocked and then incubated with the primary antibody for 30 minutes at 22°C and then with an HRP conjugated goat anti-rabbit secondary antibody.See Abreview
ab34710 staining mouse kidney sections (ab4606) by IHC-Fr. Kidney tissue was cryoprotected with 30% sucrose, sectioned using a cryostat at 10 microns and mounted onto slides. After drying overnight in fume hood, sections were fixed in 4% formalin in PBS for 10 minutes. Blocking was performed with 1% BSA for 10 minutes at 21°C. Staining with ab34710 at a 1/100 dilution in TBS/BSA with 0.02% azide was performed for 2h at 21°C. A conjugated goat anti-rabbit 594 polyclonal antibody at 1/1000 was used as the secondary antibody.See Abreview
ab34710 at 1/500 staining mouse kidney tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. An HRP conjugated goat anti-rabbit antibody was used as the secondary.See Abreview
ab34710 was used at a 1:100 dilution to detect distal tubules in normal kidney tissue.
ab34710 at 1/200 staining rat testes tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed, before a blocking step in 1% serum. The tissue was incuabted with the primary antibody for 30 minutes at 22°C and then with the HRP conjugated goat anti-rabbit secondary.See Abreview
Tissue= Human lung. 1:400 dilution of ab34710. Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative.
Immunohistochemistry of ab34710. Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow).ab34710 at 1:1250
ab34710 staining Collagen I in horse bronchial fibroblast cells by Immunocytochemistry. Cells were fixed with acetone and blocking with 3% BSA was performed for 1 hour 30 minutes at 40°C. Samples were incubated with primary antibody (1/500: in 3% BSA/ PBS) for 12 hours at 4°C. An FITC-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/160 as secondary antibody.See Abreview
Anti-Collagen I antibody (ab34710) at 1/1000 dilution + Human CollagenSecondaryDyLight™ 649 anti-rabbit secondary antibody at 1:20,000 for 30 min at RT at 1/20000 dilutionLane 1: Human Collagen Type 1. 50 ng per lane.Other Band(s): Collagen Type I splice variants and isoforms.
developed using the ECL techniquePerformed under non-reducing conditions.Detection of collagen I in Wistar rat hepatic stellate cells in GFP-transduced (left lane) and PPAR?-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 µg) was separated by native PAGE and electroblotted to nitrocellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2–2 µg/10 ml in TBS with 5% Non-fat milk.
Product References
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