Anti-Collagen I antibody [EPR7785]

• Supplier: Abcam
• Catalog: ab138492
• Prices: $395.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7785
• Applications: WB IHC-P
• Species Reactivities: Bovine, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Collagen I aa 1200-1300
• Blocking Peptide: Collagen I peptide
• Description: Rabbit Monoclonal
• Gene: COL1A1
• Conjugate: Unconjugated

Product images

Anti-Collagen I antibody [EPR7785] (ab138492) at 1/5000 dilution (unpurified) + Human skin tissue lysate at 10 µgSecondaryHRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Anti-Collagen I antibody [EPR7785] (ab138492) at 1/5000 dilution (purified) + Human skin tissue lysate at 10 µgSecondaryHRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/5000 dilution

All lanes : Anti-Collagen I antibody [EPR7785] (ab138492) at 1/1000 dilution (unpurified)Lane 1 : Human stomach tissue lysateLane 2 : Human skin lysateLane 3 : Human adrenal gland lysateLysates/proteins at 10 µg per lane.SecondaryLane 1 : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilutionLanes 2 - 3 : HRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Collagen I with unpurified ab138492 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Collagen I with purified ab138492 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Collagen I with unpurified ab138492.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Collagen I with unpurified ab138492.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

A mesenchymal glioma stem cell profile is related to clinical outcome. - A mesenchymal glioma stem cell profile is related to clinical outcome.

Balbous A, Cortes U, Guilloteau K, Villalva C, Flamant S, Gaillard A, Milin S, Wager M, Sorel N, Guilhot J, Bennaceur-Griscelli A, Turhan A, Chomel JC, Karayan-Tapon L. Oncogenesis. 2014 Mar 17;3:e91.

Hepatitis B virus infection and immunopathogenesis in a humanized mouse model: - Hepatitis B virus infection and immunopathogenesis in a humanized mouse model:

Bility MT, Cheng L, Zhang Z, Luan Y, Li F, Chi L, Zhang L, Tu Z, Gao Y, Fu Y, Niu J, Wang F, Su L. PLoS Pathog. 2014 Mar 20;10(3):e1004032.