Anti-Collagen V antibody
| Name | Anti-Collagen V antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7046 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA IHC-F IP WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Bovine, Human |
| Antigen | Full length native protein (purified) corresponding to Collagen V aa 1-1745 |
| Description | Rabbit Polyclonal |
| Gene | COL5A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody.See Abreview
ab7046 staining Collagen V in Dog skeletal muscle tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone. The primary antibody was diluted 1/100 in 10% Normal Goat Serum (ab7481) in PBS-T and incubated with the sample for 12 hours at 4°C. A Rhodamine-conjugated Goat anti-Rabbit IgG was used as the secondary antibody, diluted 1/200.See Abreview
Anti-Collagen V antibody (ab7046) at 1 µg/ml + Pancreas (Human) Tissue Lysate - adult normal tissue (ab29816) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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Ponsioen TL, van Luyn MJ, van der Worp RJ, Pas HH, Hooymans JM, Los LI. Mol Vis. 2008 Mar 26;14:652-60.
Collagen distribution in the human vitreoretinal interface. - Collagen distribution in the human vitreoretinal interface.
Ponsioen TL, van Luyn MJ, van der Worp RJ, van Meurs JC, Hooymans JM, Los LI. Invest Ophthalmol Vis Sci. 2008 Sep;49(9):4089-95.
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Guillen-Ahlers H, Buechler SA, Suckow MA, Castellino FJ, Ploplis VA. Carcinogenesis. 2008 Jul;29(7):1421-7.