Anti-Collagen VI antibody
| Name | Anti-Collagen VI antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab6588 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA IHC-F IP WB IHC-P |
| Species Reactivities | Mouse, Rat, Sheep, Rabbit, Bovine, Dog, Human, Pig, Mammalian |
| Antigen | Full length native protein (purified) corresponding to Human Collagen VI aa 1-1028 |
| Description | Rabbit Polyclonal |
| Gene | COL6A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab6588 staining Collagen VI in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and an antigen retrieval step was performed using citrate buffer. Samples were then blocked with 10% serum for 30 minutes at 20°C and then incubated with ab6588 at a 1/50 dilution for 1 hour at 37°C. The secondary used was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal (ab150078), used at a 1/500 dilution.See Abreview
ab6588 at 1/400 dilution staining Collagen VI in Human placenta (Top) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.
ab6588 staining (green) Collagen VI in a primary cell culture from Mouse retinal pigment epithelium by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody 1/500 (1% goat serum, 0.1% TX100, 1XPBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG (ab150077), dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI (blue).See Abreview
ab6588 staining Collagen VI in a primary cell culture from Pig retinal pigment epithelium by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody 1/250 (1% goat serum, 0.1% TX100, 1XPBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI (blue).See Abreview
Anti-Collagen VI antibody (ab6588) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Product References
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