All lanes : Anti-Collagen VIII alpha 1 antibody (ab100988) at 1 µg/mlLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 3 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 5 : Lung (Human) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.