Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and golgi apparatus. Recommended concentration of ab121303 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-COMMD9 antibody (ab121303) at 1/250 dilutionLane 1 : vector only transfected HEK293T lysateLane 2 : COMMD9 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cellsdeveloped using the ECL technique10-20% SDS PAGE
ab121303 at 1/100 dilution staining COMMD9 in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.