ICC/IF image of ab128893 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab128893 at 1/750 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
ab128893 staining COMP in Mouse tail osteocytes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% milk and serum for 30 minutes at room temperature; antigen retrieval was by heat mediation in a sodium citrate buffer (10mM sodium citrate, 0.05% Tween 20, pH 6.0). Samples were incubated with primary antibody (1/250 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
![All lanes : Anti-COMP antibody [EPR6289(2)] (ab128893) at 1/10000 dilutionLane 1 : Raji cell lysateLane 2 : BxPC3 cell lysateLane 3 : Saos 2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit IgG at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/715_COMP-Primary-antibodies-ab128893-1.jpg)
All lanes : Anti-COMP antibody [EPR6289(2)] (ab128893) at 1/10000 dilutionLane 1 : Raji cell lysateLane 2 : BxPC3 cell lysateLane 3 : Saos 2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit IgG at 1/2000 dilution
ab128893 at 1/10 dilution staining COMP in permeabilized Raji cells by Flow cytometry (red). Rabbit IgG negative control (green)
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD