All lanes : Anti-CoREST antibody [EPR13826(B)] (ab181139) at 1/2000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-CoREST antibody [EPR13826(B)] (ab181139) at 1/10000 dilution + MOLT4 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling CoREST with ab181139 at 1/150 (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.