Lanes 1 - 2 : Anti-CORO1B antibody (ab119714) at 1/1000 dilutionLanes 3 - 4 : Anti-CORO1B (phospho S2) antibody (ab119705) at 1/1000 dilutionLane 1 : Human A431 cells treated with Calyculin A (100 nM) for 30 minLane 2 : Human A431 cells treated with Calyculin A (100 nM) for 30 min before treatment with lambda phosphataseLane 3 : Human A431 cells treated with Calyculin A (100 nM) for 30 minLane 4 : Human A431 cells treated with Calyculin A (100 nM) for 30 min before treatment with lambda phosphatase