All lanes : Anti-CORO1B antibody [3D10] (ab119071) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CORO1B cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-CORO1B antibody [3D10] (ab119071) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab119071 at 1/100 dilution, staining CORO1B in COS7 cells transiently transfected by CORO1B by immunofluorescence.
HEK293T cells transfected with either CORO1B overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119071 at 1/100, and then analyzed by flow cytometry.