Anti-Coronin 1a antibody
| Name | Anti-Coronin 1a antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56820 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 360-462 of Human Coronin 1a |
| Description | Mouse Monoclonal |
| Gene | CORO1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Coronin 1a antibody (ab56820) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lung.
All lanes : Anti-Coronin 1a antibody (ab56820) at 1/500 dilutionLane 1 : Mouse macrophage whole cell lysateLane 2 : Mouse macrophage whole cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated Goat anti-Mousedeveloped using the ECL techniquePerformed under reducing conditions.
ab56820 staining Coronin 1a in HL-60 cells by Immunocytochemistry/ Immunofluorescence.The cells were washed three times with PBS after the phagocytosis assay and fixed with 3.3% paraformaldehyde for 15 minutes. Cells were washed twice with PBS containing 1 mm MgCl2 and 0.1% BSA (staining buffer), permeabilized with 0.2% Triton X-100 in staining buffer for 3 minutes, and blocked with PBS containing 1 mm MgCl2 and 5% BSA for 30 minutes. To assess the distribution of Coronin 1a, a phagocytosis assay was started as described above, and the cells were treated with C3bi-zymosan for 10 minutes at 37 °C. After washing twice to remove unbound C3bi-zymosan particles, the treated cells were reincubated for the indicated times at 37 °C and then fixed and permeabilized. Cells were incubated with ab56820 for 1 hour and then with an Alexa Fluor 488-conjugated phalloidin. For more details please see journal article.
Product References
Inflammatory stimuli reprogram macrophage phagocytosis to macropinocytosis for - Inflammatory stimuli reprogram macrophage phagocytosis to macropinocytosis for
Bosedasgupta S, Pieters J. PLoS Pathog. 2014 Jan 30;10(1):e1003879.
Rab27a negatively regulates phagocytosis by prolongation of the actin-coating - Rab27a negatively regulates phagocytosis by prolongation of the actin-coating
Yokoyama K, Kaji H, He J, Tanaka C, Hazama R, Kamigaki T, Ku Y, Tohyama K, Tohyama Y. J Biol Chem. 2011 Feb 18;286(7):5375-82.